aavs1 safe harbor locus (Addgene inc)
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aavs1 safe harbor locus
Aavs1 Safe Harbor Locus, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 79 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aavs1+safe+harbor+locus/AAVS1-TALEN-L+(Plasmid+%2359025)/pmc12017769-286-9-36
Average 95 stars, based on 79 article reviews
Aavs1 Safe Harbor Locus, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 79 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aavs1+safe+harbor+locus/AAVS1-TALEN-L+(Plasmid+%2359025)/pmc12017769-286-9-36
Average 95 stars, based on 79 article reviews
aavs1 safe harbor locus - by Bioz Stars,
2026-09
95/100 stars
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Plasmid Preparation:Article Title: Prediction and design of transcriptional repressor domains with large-scale mutational scans and deep learning Article Snippet: .. Briefly, the pEF reporter was inserted at the Article Title: Genetic modifiers of somatic expansion and clinical phenotypes in Huntington’s disease reveal shared and tissue-specific effects Article Snippet: The entire HTT exon 1 coding sequence was amplified using UltraRun® LongRange PCR Kit (QIAGEN, 206442) with 10% DMSO using the following PCR conditions: denaturation 93°C (3 min), 35 cycles of 93°C (30 s), 61°C (15 s), 68°C (60 s), with a final extension of 72 °C (10 min), using primers with SalI cloning sites (F: CATGTACGgtcgacaccgccATGGCGACCCTGGAAAAGCTG, R: CATGTACGgtcgacTCGGTGCAGCGGCTCCTC). .. Amplicons were cloned into a plasmid for targeting the Article Title: SFSWAP is a negative regulator of OGT intron detention and global pre-mRNA splicing Article Snippet: .. The reporter construct generated above was integrated into the Article Title: SFSWAP is a negative regulator of OGT intron detention and global pre-mRNA splicing Article Snippet: .. The reporter construct generated above was integrated into the Clone Assay:Article Title: Genetic modifiers of somatic expansion and clinical phenotypes in Huntington’s disease reveal shared and tissue-specific effects Article Snippet: The entire HTT exon 1 coding sequence was amplified using UltraRun® LongRange PCR Kit (QIAGEN, 206442) with 10% DMSO using the following PCR conditions: denaturation 93°C (3 min), 35 cycles of 93°C (30 s), 61°C (15 s), 68°C (60 s), with a final extension of 72 °C (10 min), using primers with SalI cloning sites (F: CATGTACGgtcgacaccgccATGGCGACCCTGGAAAAGCTG, R: CATGTACGgtcgacTCGGTGCAGCGGCTCCTC). .. Amplicons were cloned into a plasmid for targeting the Article Title: An optogenetic method for the controlled release of single molecules. Article Snippet: For the mScarlet-CD4-PhoCl-RER construct used in stable cell generation for the UV dose–response assay, fragments encoding the TRE promoter, amplified from vector pTREtight2, and mScarletCD4-PhoCl-RER were cloned into AAVS1 Safe Harbor Targeting Knock-in HR Donor 2.0 (System Bioscience, no. GE622A-1) using NEBuilder HiFi DNA Assembly (New England Biolabs) following digestion of the vector with restriction enzymes SpeI and MluI. .. The guide RNA sequence targeting the Article Title: An optogenetic method for the controlled release of single molecules Article Snippet: For the mScarlet-CD4-PhoCl-RER construct used in stable cell generation for the UV dose–response assay, fragments encoding the TRE promoter, amplified from vector pTREtight2, and mScarlet-CD4-PhoCl-RER were cloned into AAVS1 Safe Harbor Targeting Knock-in HR Donor 2.0 (System Bioscience, no. GE622A-1) using NEBuilder HiFi DNA Assembly (New England Biolabs) following digestion of the vector with restriction enzymes SpeI and MluI. .. The guide RNA sequence targeting the Sequencing:Article Title: An optogenetic method for the controlled release of single molecules. Article Snippet: For the mScarlet-CD4-PhoCl-RER construct used in stable cell generation for the UV dose–response assay, fragments encoding the TRE promoter, amplified from vector pTREtight2, and mScarletCD4-PhoCl-RER were cloned into AAVS1 Safe Harbor Targeting Knock-in HR Donor 2.0 (System Bioscience, no. GE622A-1) using NEBuilder HiFi DNA Assembly (New England Biolabs) following digestion of the vector with restriction enzymes SpeI and MluI. .. The guide RNA sequence targeting the Article Title: An optogenetic method for the controlled release of single molecules Article Snippet: For the mScarlet-CD4-PhoCl-RER construct used in stable cell generation for the UV dose–response assay, fragments encoding the TRE promoter, amplified from vector pTREtight2, and mScarlet-CD4-PhoCl-RER were cloned into AAVS1 Safe Harbor Targeting Knock-in HR Donor 2.0 (System Bioscience, no. GE622A-1) using NEBuilder HiFi DNA Assembly (New England Biolabs) following digestion of the vector with restriction enzymes SpeI and MluI. .. The guide RNA sequence targeting the Construct:Article Title: SFSWAP is a negative regulator of OGT intron detention and global pre-mRNA splicing Article Snippet: .. The reporter construct generated above was integrated into the Article Title: SFSWAP is a negative regulator of OGT intron detention and global pre-mRNA splicing Article Snippet: .. The reporter construct generated above was integrated into the Generated:Article Title: SFSWAP is a negative regulator of OGT intron detention and global pre-mRNA splicing Article Snippet: .. The reporter construct generated above was integrated into the Article Title: SFSWAP is a negative regulator of OGT intron detention and global pre-mRNA splicing Article Snippet: .. The reporter construct generated above was integrated into the Zinc-Fingers:Article Title: Telomeric DNA breaks in human induced pluripotent stem cells trigger ATR-mediated arrest and telomerase-independent telomere damage repair. Article Snippet: DD-ER-TRF1-FokI (lacking the mCherry sequence present in the original construct) was PCR amplified and Gibson cloned into Addgene plasmid #22074 downstream of the TRE promoter in place of the EGFP reporter. .. For targeted integration of rtTA and DD-ER-TRF1-FokI into the |